Short Courses

Half day short courses are offered for an additional fee of $45 per person and will be held on Sunday afternoon, May 21, 2023, 2:00 - 5:00 p.m.

1. Introduction to Capillary Liquid Chromatography

Course Instructors:

Justin Godinho, GlaxoSmithKline
James Grinias, Rowan University

Course Description:

This course is designed to introduce those familiar with analytical scale HPLC to capillary (or “nano”) liquid chromatography. Although both techniques are based on the same fundamental principles, capillary LC has a number of distinct advantages and challenges that will be detailed. Commercial instrument options, as well as the basics of preparing your own capillary LC columns, will be described. Because one of the most prominent uses of capillary LC is its coupling to mass spectrometry for complex biological sample analysis, special attention will be given to this important area. Both academic and industrial researchers will be able to apply the information gained through this course to overcome the challenges faced when using this essential technique.
After completing this course, participants will be able to understand the differences between analytical and capillary scale LC and describe the fundamentals of capillary LC column preparation. They will also be able to determine the best detection modes for a given application and explain the advantages of coupling capillary LC with mass spectrometry and how to approach method development using capillary LC-MS. Finally, they will be able to identify best practices for the use of capillary LC to solve analytical challenges.


James Grinias is an Associate Professor in the Department of Chemistry & Biochemistry at Rowan University in Glassboro, NJ. Dr. Grinias has a number of research interests focused on chemical separations and microfluidics, both at the fundamental level and for the analysis of biological systems. He is the recipient of the 2021 American Chemical Society Satinder Ahuja Award for Young Investigators in Separation Science and the 2022 LCGC Emerging Leader in Chromatography. At Rowan, he teaches courses in general, analytical, and bioanalytical chemistry while also conducting research on UHPLC column performance and instrument miniaturization.

Justin Godinho is an Investigator at GlaxoSmithKline in Upper Merion, PA. Dr. Godinho’s research interests largely focus on the fundamentals of chromatographic separations in capillary ultrahigh pressure liquid chromatography columns. His research has explored methods of capillary column packing, column characterization and column implementation. He has been involved in collaborations studying the microstructure of the packed bed and how it relates to column performance. During his postdoctoral research at the University of North Carolina at Chapel Hill he studied electrophoretic separations in microfluidic devices. These devices were coupled with mass spectrometry for analyte detection.

2. Fundamentals and Applications of Ion Mobility Spectrometry

Course Instructors:

Christian Bleiholder, Florida State University
Fanny Caroline Liu, Florida State University

Course Description:

This course is designed to describe the fundamental and applied aspects of ion mobility spectrometry / mass spectrometry (IMS/MS). The course will start with fundamentals of the separation process in IMS and the coupling with MS. This will be followed by a discussion of the various IMS/MS technologies currently commercially available. Subsequently, the course will discuss case studies that apply these IMS/MS technologies to challenging analytical problems. Academic and industrial researchers will be able to apply the information gained from this course to realize the potential of this powerful separation dimension. After completing this course, participants will be able to understand the difference between several types of ion mobility instruments, describe the fundamentals of ion mobility separations, and identify best practices for the use of IMS/MS to solve analytical challenges.


Christian Bleiholder is an Associate Professor of Chemistry and Biochemistry at the Florida State University. He pursued his D.Sc. degree in Chemistry with Profs Rolf Gleiter and Sandor Suhai (University of Heidelberg & German Cancer Research Center, 2007) and his post-doctoral research with Michael T. Bowers (University of California, Santa Barbara, 2013). Current research focuses on trapped ion mobility spectrometry and analysis of ion mobility data to elucidate protein structures. His awards include a fellowship from the Alexander-von-Humboldt Foundation (2008–2010), a Postdoctoral Research Award from the American Chemical Society (2011), and a CAREER award from the National Science Foundation (2017).

Fanny Caroline Liu received her PhD in Applied Physical Chemistry from the University of Heidelberg in Heidelberg/Germany, studying optical biosensors. Dr. Liu joined the Bleiholder Laboratory at Florida State University in 2014 as a postdoctoral researcher to develop a tandem-trapped ion mobility spectrometer / mass spectrometer (tandem-TIMS/MS). Currently, she is a research faculty in the Bleiholder Laboratory. Dr. Liu is co-inventor of the tandem-TIMS technology (US patent 10794861, 2020/10/6). Her current research interests involve the native MS applications of tandem-TIMS/MS and implementation of ion activation methods in tandem-TIMS/MS such as collisional-induced dissociation (CID) and UV photodissociation (UVPD) for top-down protein analysis.

3. Capillary Electrophoresis-Mass Spectrometry for Metabolomics: Principles and Applications

Course Instructor:

Rawi Ramautar, Leiden University, the Netherlands

Course Description:

The main objective of this short course is to provide an overview of the main capillary electrophoresis-mass spectrometry (CE-MS) approaches used in metabolomics, including their working/separation mechanisms. The course shows in particular the applicability of CE-MS for volume-restricted biological samples and for compound classes that are (still) difficult to analyze with chromatographic-based separation techniques. Ample attention is devoted to the coupling of CE to MS using both the classical and the recently developed interfacing designs. The reproducibility of CE-MS for metabolomics studies is also considered by highlighting the Metabo-Ring trial.
Advances and utility of CE-MS-based metabolomics is demonstrated by discussing a few recent studies in detail. For example, it is shown that neurotransmitters can be directly analyzed in rat microdialysis samples without using derivatization and sample pretreatment. Highly polar metabolites, such as ATP, ADP and AMP, can be analyzed in extracts from just a limited number of mammalian cells, opening up the possibility to assess the adenylate energy charge in studies dealing with microscale cell cultures. This course is given in an interactive way by using tools such as for example the Mentimeter.


Rawi Ramautar obtained his PhD on the development of capillary electrophoresis-mass spectrometry methods for metabolomics from Utrecht University, the Netherlands, in 2010. Intrigued by metabolomics for disease prediction and diagnosis, Rawi switched to the Leiden University Medical Center to broaden his horizon on this topic. In 2013 and 2017, he received the prestigious Veni and Vidi research grants from the Netherlands Organization for Scientific Research for the development of CE-MS approaches for volume-restricted metabolomics. Currently, he is an associate professor at the Leiden University where his group is developing microscale analytical workflows for sample-restricted biomedical problems. Rawi Ramautar was recently selected for the Top 40 under 40 Power List of the Analytical Scientist. He is editor of Microchemical Journal (Elsevier).